Of this squad list, four will be cut for the final group before the World Cup kicks off in June.
Manuel Neuer has been included in Germany's preliminary squad for the World Cup, with Joachim Low including four goalkeepers in an initial 27-man selection.
The 32-year-old could make a high-profile return in Bayern's DFB-Pokal showdown with Eintracht Frankfurt on Saturday, while Germany have scheduled pre-tournament friendlies against Austria and Saudi Arabia.
In a major surprise, World Cup-winning goalscorer Mario Gotze has been dropped from Germany's squad for the mega event.
"Neuer is back in full training and we want to see and judge him in the training camp", said Low, who has named Barcelona's Marc-Andre ter Stegen, Bayer Leverkusen's Bernd Leno and Kevin Trapp of Paris Saint-Germain as his other goalkeepers. "We saw that we're not as good as we're always told we are - and perhaps some of us think we are".
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Freiburg striker Nils Petersen earned his first call-up to the national team, ensuring his place in the squad ahead of Bayern Munich forward Sandro Wagner.
Cologne defender Jonas Hector has been included despite his club side being relegated from the Bundesliga this season.
Gotze, 25, netted the only goal as Germany beat Argentina 1-0 after extra time in the final of the 2014 FIFA World Cup at Rio de Janeiro's Maracana, Brazil, but he has not convinced Low he is worthy of a place in the squad this year.
They open their World Cup campaign against Mexico on June 17, before fixtures against Sweden (June 23) and South Korea (June 27).